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Applications

Forensics

Enhanced accuracy and sensitivity for analyzing degraded DNA and complex mixtures, incorporating key markers for SNP genotyping and tissue typing in a single panel.

Image by George Prentzas

Happening Right Now

Researchers from the Swedish National Forensic Centre have evaluated Hyperplex PCR™ (hpPCR™) for mRNA-based cell type identification. Their presentation will delve into a novel Hyperplex PCR assay and its practical application in identifying cell types within complex forensic samples.

Benefits of Hyperplex PCR for Enhanced Forensic Analysis

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  • No crosstalk/interference between targets

  • Push sensitivity beyond legacy PCR methods, towards single molecule detection

  • Same-day results with streamlined workflow

  • mRNA profiling for tissue type identification

  • SNP genotyping for human identification

  • Design panels with up to 100 targets (50 pairs of SNPs)

Resistance to inhibitors

The hpPCR assay is inherently more resistant to inhibitor effects due to the optimized sample handling, resulting in minimal impact on signal intensity.

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This enhanced sensitivity surpasses that of digital PCR, while the dual-layer molecular specificity—achieved through both PCR and padlock probes—ensures no false amplification, even at high cycle numbers.

Implement Hyperplex PCR in your forensic analysis

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What type of quantification needs do you have
Do you require SNP specificity?
Do you have PCR primers optimized for your targets?

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